4. Remove the sheath container lid.
Unscrew the clamp knob and push down to loosen, if necessary. Tilt the cap to the side to remove it from the
tank.
5. Add 10L of sheath fluid, such as BD FACSFlow™ solution, to the sheath container.
Note: The 10L will reach the interior line on the sheath tank. Do not fill the sheath tank further.
6. Replace the sheath container lid.
7. Reconnect the green air line and the alarm line.
8. Make sure the gasket on the inside lip of the sheath lid is seated correctly and has not slipped out of
position.
If the gasket is not seated correctly, the tank will not pressurize properly.
9. Close the sheath lid and tighten the clamp knob to finger-tight. Ensure that the blue sheath fluid line is not
kinked.
More information
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Removing air bubbles (page 27)
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Changing the sheath filter (page 41)
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Cleaning or replacing the sheath gasket (page 45)
Removing air bubbles
Introduction
This topic describes how to remove trapped air bubbles in the sheath filter and the sheath line. Air bubbles can
occasionally dislodge and pass through the flow cell, resulting in inaccurate data.
Note: Perform this activity every time the sheath tank is refilled.
Chapter 3 Cytometer setup 27
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