No events in acquisition display and RUN button is green
Possible causes Recommended solutions
Threshold is not set to the
correct parameter (usually FSC)
Set the threshold to the correct parameter for your application.
Threshold level is too high Lower the threshold level.
PMT voltage for threshold
parameter is set too low
Set the PMT voltage higher for the threshold parameter.
Gating issue See the BDFACSDiva™ Software Reference Manual for information on setting gates.
Air in the sheath filter Purge the filter. See Removing air bubbles (page 27).
Air bubble or debris in the flow
cell
Prime the fluidics system. See Preparing the fluidics (page 31).
No sample in the tube Verify that sample remains in the tube and if necessary, add sample to the tube or
install a new sample tube.
Sample is not mixed properly Mix the sample to suspend the cells.
Waste tank is full Empty the waste tank.
PMT voltages set too low or too
high for display parameter
Adjust the PMT voltages.
Too few events are displayed Increase the number of events to display.
Sample injection tube is clogged Remove the sample tube to allow backflushing.
If the event rate is still erratic, clean the sample injection tube. See Cleaning the
fluidics (page 37).
Bal seal is worn Replace the Bal seal. See Changing the Bal seal (page 42).
Instrument is not warmed up Wait 30 minutes for the instrument to warm up.
Laser delay is set incorrectly Adjust the laser delay settings. See Manual settings (page 93).
Laser is not functioning Verify the malfunction by changing the threshold to an alternative laser while running
the appropriate sample. If unsuccessful, contact BDBiosciences.
Tube is cracked or misshapen Replace the sample tube.
Chapter 9 Troubleshooting 105
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