BD FACSymphony A5 SE - Page 39

Procedure
To perform a system flush:
1. Remove the sheath filter.
a. Press the quick-disconnects on both sides of the filter assembly.
b. Remove the filter assembly.
c. Connect the two fluid lines.
Do not run detergent, bleach, or ethanol through the sheath filter. They can break down the filter paper
within the filter body, causing particles to escape into the sheath fluid, possibly clogging the flow cell.
2. Empty the sheath container and rinse it with DI water.
3. Fill the sheath container with at least 1 L of undiluted BDFACSClean™ solution.
4. Empty the waste container, if needed.
5. Open the roller clamp by the fluidics interconnect, and drain the fluid into a beaker for 5seconds.
6. Remove the DI water tube from the SIP.
7. Prime the instrument twice:
a. Press the PRIME button on the fluidics control panel.
b. When the STANDBY button lights (amber), press the PRIME button again.
8. Install a tube with 3mL of undiluted BDFACSClean™ solution on the SIP and put the tube support arm
underneath the tube.
See Maintenance overview (page 36) for other recommended cleaning solutions.
9. Press RUN and HIGH on the cytometer fluid control panel. Run for 30 minutes.
10. Press the STANDBY fluid control button and depressurize the sheath container by lifting the vent valve.
11. Empty the waste container to avoid the mixing of bleach and BD
®
Detergent Solution Concentrate.
Do not mix BD
®
Detergent Solution Concentrate and bleach because they produce chlorine gas.
12. Repeat steps 2 through 10 with 1.5% dilution of BD
®
Detergent Solution Concentrate.
Note: The BD
®
Detergent Solution Concentrate must be diluted before use. Mix one full 15mLbottle of
BD
®
Detergent Solution Concentrate into 985mL of DI water to make 1L total.
13. Leave the system filled with 1.5% dilution of BD
®
Detergent Solution Concentrate overnight to soak (if
possible, up to 18 hours) to reduce air adhesion throughout the system. When soaking, the system power
should be disconnected and secured. Upon completion of the soak period, restore power and continue to the
next step in this procedure .
14. Repeat steps 2 through 10 with DI water.
15. Replace the sheath filter and refill the sheath container with sheath fluid.
Chapter 4 Maintenance 39