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Measuring Instruments
FACSymphony A5
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BD FACSymphony A5 - Recording and analyzing data
128 pages
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6
Recording and analyzing data
This chapter covers
the followin
g topics:
•
Data recording an
d analysis workflo
w (page
88)
•
Preparing the workspace (page
89)
•
Recording data (page
90)
•
Analyzing data (page
93
)
•
Reusing an analysis (page
99)
86
88
Table of Contents
Main Page
Default Chapter
5
Table of Contents
5
Chapter 1: About this Guide
9
What this Guide Covers
10
Conventions
11
About the Documentation
11
Instrument Technical Support
13
Chapter 2: Introduction
15
System Overview
16
Cytometer Overview
17
Control Panel
20
Fluidics System
21
Sheath and Waste Containers
28
Optics
29
Workstation
31
Chapter 3: Cytometer Setup
33
Starting the Cytometer and Computer
34
Preparing the Sheath Container
35
Removing Air Bubbles
37
Preparing the Waste Container
40
Priming the Fluidics
42
About the Optical Filters and Mirrors
43
Custom Configurations and Baselines
45
Chapter 4: Maintenance
47
Maintenance Overview
48
Cleaning the Fluidics
49
Shutting down the Cytometer
51
Flushing the System
52
Replacing the Waste Container Cap
54
Changing the Sheath Filter
56
Changing the Bal Seal
58
Changing the Sample Tube O-Ring
60
Cleaning or Replacing the Sheath Gasket
62
Chapter 5: Optimizing Cytometer Settings
63
Cytometer Settings Workflow
64
Verifying the Configuration and User Preferences
66
Running a Performance Check
68
Setting up an Experiment
72
Creating Application Settings
78
Recording Compensation Controls
81
Calculating Compensation
84
Chapter 6: Recording and Analyzing Data
87
Data Recording and Analysis Workflow
88
Preparing the Workspace
89
Recording Data
90
Analyzing Data
93
Reusing an Analysis
99
Chapter 7: Troubleshooting
101
Cytometer Troubleshooting
102
Electronics Troubleshooting
110
Chapter 8: Manual Settings
111
About Laser Delay
112
Optimizing Laser Delay
113
Adjusting Area Scaling
115
Chapter 9: Supplies and Consumables
121
Ordering Information
122
Beads
122
Reagents
123
Equipment
124
Index
125
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