Chapter 5: Optimizing cytometer settings 65
Workflow for
optimizing settings
Cytometer optimization consists of the following steps.
Note: Application settings are optional and do not have to be
saved for the experiments. However, they are useful for optimizing
cytometer settings.
About the
examples
The examples in this chapter use a 4-color bead sample with the
following fluorochromes:
•FITC
•PE
• PerCP-Cy™5.5
•APC
If you follow this workflow with a different bead sample (or
another sample type), your software views, data plots, and
statistics might differ from the example. Additionally, you might
need to modify some of the instructions in the procedure.
The information shown in italics is for example only. You can
substitute your own names for folders and experiments.
Step Description
1 Verifying the configuration and user preferences (page 66)
2 Running a performance check (page 68)
3 Setting up an experiment (page 72)
4 Creating application settings (page 78)
5 Recording compensation controls (page 81)
6 Calculating compensation (page 84)
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