User’s Guide80
6. Verify that the positive populations are on scale.
If a positive population is off scale, lower the PMT voltage for
that parameter until the positive population can be seen
entirely on scale.
7. Unload the multicolor sample.
8. Place a tube containing DI water on the SIP and put the
cyto
meter on standby.
9. (Optional) Save the application settings by right-clicking
Cyt
ometer settings in the Browser, then selecting Cytometer
Settings > Save.
10. In the Sa
ve Cytometer Settings dialog, enter a descriptive name
for the settings.
11. Click OK.
The application settings are saved to the catalog.
Next step Recording compensation
controls (page 81)
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