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Essen BioScience IncuCyte ZOOM - User Manual

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IncuCyte™ ZOOM Users Manual
2013A
Essen BioScience, Inc.
www.EssenBioScience.com

Table of Contents

Questions and Answers

  • A
    Alexis ToddAug 2, 2025
    Why are my Essen BioScience IncuCyte ZOOM images blurry?
    • M
      Max CurryAug 2, 2025
      Blurry images can occur if the incubator containing your IncuCyte™ ZOOM is not on a sturdy stand, as vibration can negatively affect image quality. Scheduling scans when the incubator will not be accessed may also help, since opening and closing the door can cause image blurring.
  • D
    dianecoxAug 8, 2025
    How to fix focus issues caused by air bubbles in Essen BioScience IncuCyte ZOOM Laboratory Equipment?
    • K
      Kelli WeeksAug 8, 2025
      To resolve focus issues caused by air bubbles, manually remove the air bubbles to obtain desired images. You can also modify your pipetting technique or use a de-bubbler before placing vessels in the IncuCyte™ ZOOM.
  • R
    richardssaraAug 14, 2025
    How to fix out of focus fluorescent cells using Essen BioScience IncuCyte ZOOM Laboratory Equipment?
    • E
      Erica EllisAug 14, 2025
      If you're planning to image in fluorescence with a 20X objective, investigate the best setting for your system and vessel. Adjusting the corrective ring to 1.4µM may be the optimal setting.
  • J
    Jason ReynoldsAug 20, 2025
    What to do if edges of wells are out of focus in Essen BioScience IncuCyte ZOOM Laboratory Equipment?
    • K
      Kelly CarsonAug 20, 2025
      To eliminate visual edge effects, increase the total media volume in each well. Sufficient proliferation data can still be generated with only 100 µL in each well.
  • C
    Christopher EvansAug 26, 2025
    What to do if there are striations and scratches on Essen BioScience Laboratory Equipment?
    • L
      Lori SanchezAug 26, 2025
      If striations and scratches are visible on the bottom of the well, and this is a recurring problem or affects data analysis, consider using a different vessel.

Summary

Chapter 1: Warranty

1.1 Limitation of Warranty

Details warranty exclusions and limitations, including maintenance and misuse.

1.3 Warnings and Disclaimers

Lists actions that may void warranty and general responsibility disclaimers.

Chapter 2: Getting to know your IncuCyte ZOOM

2.3 Safety has Priority

Provides essential safety guidelines for operating the IncuCyte™ ZOOM system.

2.5 Unpacking and Checking the IncuCyte

Outlines the process for unpacking and inspecting the IncuCyte™ ZOOM system components.

Chapter 3: Installation of the IncuCyte ZOOM Hardware

3.2 Removing the Shipping Pin

Step-by-step guide for removing the shipping pin before system installation.

3.3 Installing the Filter Module

Instructions for correctly installing the filter module into the IncuCyte™ microscope.

3.4 Installing an Objective

Details on how to install different objective magnifications for the IncuCyte™ ZOOM.

3.6 Placing the Microscope into the Incubator

Guide on correctly positioning the microscope unit inside the incubator.

Chapter 4: Establishing Network Connections

4.1 DHCP Based Networks

Procedure for connecting the controller to a network using DHCP for automatic IP assignment.

4.2 Static IP Based Networks

Steps to reconfigure the controller for a static IP address on a fixed IP network.

Chapter 5: Installation of the IncuCyte Control Software

5.1 Software Installation: Windows XP

Instructions for installing the control software on Windows XP operating systems.

5.3 Connecting

Covers initial connection procedures, including setting date and time.

5.3.2 Establishing User Accounts

Guide on creating and managing user accounts with different permission levels.

Chapter 6: Calibrating the IncuCyte ZOOM System

6.1 System Calibration

Procedure to ensure accurate and repeatable microscope motion for image acquisition.

6.2 Fluorescence Calibration

Steps for performing fluorescence calibration using the provided kit and slides.

Chapter 7: Cell Culture Monitoring and Data Analysis

7.2 User Interface

Overview of the IncuCyte™ software interface and navigation.

7.3 Scheduling Scans and Loading Vessels

Guide to initiating assays by scheduling scans and loading vessels.

7.4 Finding and Viewing Scanned Vessels

Methods for searching and viewing acquired scan data for specific vessels.

7.5 Data Processing

Explains the software's modes for analyzing and summarizing qualitative images into quantitative data.

7.6 Graphing and Exporting Data

Instructions on generating graphs and exporting analysis results.

7.7 Exporting Images and Movies

Details on how to export images and create movies from acquired data.

Chapter 9: Troubleshooting

9.1 Images and What they Mean

Explains common image problems and their potential solutions for better analysis.

9.2 Contacting Technical Support

Instructions on how to export log files to aid technical support in resolving issues.

Chapter 10: Performance Optimization

Essen BioScience IncuCyte ZOOM Specifications

General IconGeneral
ModelIncuCyte ZOOM
TypeLive-cell imaging system
Imaging ModalityPhase contrast and fluorescence
Fluorescence ChannelsUp to 4 channels
Objective Magnification10x
Temperature ControlAmbient to 40°C
O2 ControlNo
Vessel CompatibilityMicroplates (6- to 384-well), flasks, and dishes
Image AnalysisIntegrated
Data OutputTime-lapse images and quantitative data
SoftwareIncuCyte Software
CompatibilityWindows
Weight27.2 kg
IncubationYes