The Tiled Field of View (FOV) is available when scanning Corning 96 well plates at 10X.
If a 96-well plate is selected as the vessel type, the Scan Type drop-down box located
under the Scan Mode in the Scan Setup tab will include a “Tiled Field of View” option.
NOTE: The 10X FOV is similar to imaging a 96-well plate with a 4X objective in standard
mode.
8.4 NeuroTrack
TM
Module
The NeuroTrack™ Module is used to identify neurite dynamics for the study of neurotoxicity,
neurodegeneration, neuronal development, and differentiation. The NeuroTrack™ Module can
quantify multiple label-free assay metrics such as neurite length, cell body clusters, and branch
points. In the first step of the process, the NeuroTrack™ software analyzes a HD phase image of
neurons by first differentiating the cell bodies from the background based on texture and/or
brightness, and masks them as cell body clusters. From here, linear features are detected based
on width and brightness and are masked as neurites. The segmentation mask can be refined and
filtered by adjusting a few parameters to tailor the mask to the specific cell type used in the
assay.
Figure 30. Diagram of Tiled Field of View
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