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Essen BioScience IncuCyte ZOOM - 7.3 Scheduling Scans and Loading Vessels

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Page 29
The IncuCyte™ ZOOM Software was designed to allow Users to efficiently navigate throughout
the program by clicking on text as well as graphical icons and images. Users should take the time
to investigate the functionality of the IncuCyte™ software interface as well as moussing over the
information icons found throughout the window screens.
7.3 Scheduling Scans and Loading Vessels
To initiate any assay, begin by selecting the Schedule Scans option located on the
left side task list. Once selected, the scheduling interface will enable the User to either schedule
scans on a 24-Hour Repeating schedule or a single Scan on Demand.
7.3.1 24-Hour Repeating
The 24-Hour Repeating Schedule allows Users to define a set interval for acquisition
times. Once the 24-Hour Repeating vertical tab is selected, you will be required to enter
information that defines your specific assay.
7.3.1.1 Vessel selection: In the Drawer Setup pane, determine which tray position
(front, middle or rear) you would like to place your vessel and then use the
“Add Vessel” button to select a vessel type.
1. Once the vessel selection window is open, you will be able to
search a table for your vessel of choice. The IncuCyte™ software
automatically recognizes the tray type required for each vessel and
will display the tray type below the vessel selection table.
2. At this time, highlight an empty vessel position within the tray and
click the “OK” button. Once the vessel selection is completed the
Scan Setup and vessel Properties tabs become activated.
7.3.1.2 Scan Setup Tab: At this time select the channels required for image
acquisition of your vessel, as well as the scan mode, scan pattern, spectral
un-mixing, and analysis job setup. Refer to Table 2 for details.

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