Table 2. Scan Set-Up
Select phase, green, or red or a combination of the three.
NOTES: Acquisition time is the amount of time that the LED will
illuminate the specimen. The default acquisition times for the green
and red channels routinely work well. If phototoxicity is a problem,
lower the acquisition times. If very faint objects are being imaged on a
low background (objects and background together less than 3 CU) it
may be helpful to increase the acquisition time.
Scan Type: For most vessel types, the software will automatically
choose a standard scan type. Special scan types are available when
Essen Bioscience Image Lock plates are selected, or when special
module have been purchased (e.g. Angiogenesis or Scratch Wound).
Scan Pattern: The Scan Pattern describes the number and locations of
images that are generated for each well (microplates) or sector
(flask/dish). The Scan Pattern is displayed within the software as a
pattern of green squares, where each square represents one image.
Create new scan patterns by using the edit scan patterns button on the
lower left corner of the screen. Scan Patterns can be edited or created
for all 3 magnifications, however, only the scan pattern that matches
the current IncuCyte™ ZOOM configuration can be chosen when
scheduling a scan.
May be required to remove fluorophores that produce signal in both
color channels. Un-mixing can also be completed in the Vessel View
(Refer to section 7.4.2.4 for more information on spectral unmixing).
Once parameters are defined for your specific assay type, cell type, and
magnification Users can apply those to future assays at the time of
assay scheduling. You must complete an image collection and
processing definition at least once during assay development before
launching an analysis job setup during the scheduling step. Refer to
Analysis Job Rules in Established Mode (section 7.5.2.3).
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