Chapter 8: Special Modules and Scan Types
In addition, to the Standard Basic Analyzer, special Image Processing Software Modules are available with
enhanced processing features. These Modules allow Users to customize processing for migration and
invasion (Scratch Wound), angiogenic tube formation (Angiogenesis), and neurite dynamics
(NeuroTrack™).
The Invasion/Migration and Angiogenesis (at 10X objective) require additional scan types other than the
standard scanning types used for HD phase-contrast, green channel fluorescence and red channel
fluorescence imaging. Using Essen ImageLock™ plates, Users have the ability to analyze invading or
migrating cells using the Scratch Wound scan type. When users want to visualize a larger, more
integrated view of the angiogenic network without compromising image resolution, the Tiled Field of
View (FOV) mosaic image scan type option is available when using a 10X objective.
Special Modules and Scan Types are described in further detail below.
8.1 Scan Types available with Essen ImageLock™
Essen BioScience manufactures a special 24 and 96-well plate type called the Essen ImageLock™
plate. These plates facilitate very precise, repeated imaging within each well. Under standard
imaging conditions, slight variations can occur in the location of imaging from one scan to the
next, resulting in small jumps between frames. When very precise, repeated imaging is required –
for instance, when generating a movie – use the Essen ImageLock™ plate. When utilizing an
ImageLock™ plate, Users can be run either the Scratch Wound or Standard (with lock) scan type
options.
Because the scanning process is so precise in ImageLock™ mode, the scan length will be longer
than that for a plate run in Phase-Contrast mode.
The scan patterns available for the ImageLock™ 24 and 96-well plates differ from those available
for standard 24 and 96-well plates. A maximum of three images is possible per well and the scan
pattern is arranged in a straight line through the center of each well, dictated by the grid on the
bottom of the wells.
8.1.1 Standard (with Lock)
By selecting an ImageLock™ plate and running it in Standard (with lock) mode, the
highest fidelity movie will be generated. When an ImageLock™ plate is selected as the
vessel type , the Scan Type drop-down box located under the Scan Mode in the Scan
Setup tab will include the Standard (with lock) option.
8.1.2 Scratch Wound
The grid on the bottom of the wells of an Essen ImageLock™ plate allows the IncuCyte™
ZOOM to find an exact location on the plate for imaging, which is necessary when
performing migration and invasion assays using the Essen Woundmaker™. When an
ImageLock™ plate is selected as the vessel type, the Scan Type drop-down box located
under the Scan Mode in the Scan Setup tab will include the Scratch Wound option.
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