7.10.1 Selecting Wells
To add “well items” (cells, compounds, etc.) to a well or grouping of wells, the desired
area must first be selected on the plate layout. Left-click on a well, or click-and-drag over
a group of wells, to select a specific area of the plate in which to work. Wells may be
deselected in the same manner. To easily select or deselect entire rows or columns, click
the row/column label along the border of the plate. Select and deselect all wells in the
plate by clicking on the “All” button on the top-left of the plate. Immediately to the left
of the “All” button is a “blank plate” icon that will also deselect all wells.
7.10.2 Adding Well Items
Before any information can be applied to a well on the plate map, it must first be loaded
into one of three lists to the left of the plate map. “Compounds” are any treatments to a
well where concentrations or dilutions must be specified. “Cells” specifies type, passage,
and seeding density for cells present on the plate. “Growth Conditions” are additions
such as growth medium where concentrations of a reagent will not vary or need not be
specified on the plate map.
7.10.2.1 Compounds
1. To add a compound to the plate map, select the “Compound” tabs then
click the “New” button below the (blank) compound list.
2. In a new window, enter a name to appear on the plate map and,
optionally, a longer description to appear in the list of compounds.
Additionally, select a color in which the compound will appear on the
plate map; when creating a dilution of a compound, higher
concentrations will appear as darker shades of the indicated color.
3. After creating a compound description, select the desired area of the
plate and click “Add ___...”.
4. A new window will appear allowing the User to select a desired
concentration and, optionally, a dilution series. First, select the units in
which concentration will appear by clicking the drop-down menu at the
top of the window (the default setting is “mg/mL”) and enter a numerical
concentration to the left. If only a single concentration is desired, click
“OK” and the compound will appear on the plate map.
5. To create a dilution series, check the box next to “Create a dilution” to
activate dilution options. In the drop-down menu immediately to the
right, select “divide by” or “subtract” and enter value by which to dilute.
Alternatively, select “manually enter” to enter a range of concentrations
separated by spaces or semicolons (1000; 300; 100; 30…). To arrange the
dilution series on the plate, select the direction in which the dilution
series runs by selecting a direction under “Dilute wells…” and the
direction in which the replicates run. If, for example, a dilution series
runs from left-to-right and the User wants the replicates to be arranged
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