horizontally, a box will appear allowing the User to specify how many
wells exist at each concentration (singlets, duplicates, triplicates, etc.).
Once the dilution series is specified, click “OK” and the compound will
appear on the plate map in the specified series.
7.10.2.2 Cells
To add a cell type, select the “Cells” tab then click the “New” button. Once a
region of the plate is selected, click “Add ___...” to open up a new window.
Here, specify passage number and seeding density, and click “OK” to add the
cell type to the plate map.
7.10.2.3 Growth Conditions
Once a growth condition is specified in the list, simply click “Add ___...” to
add it to the plate. Because the Growth Conditions category is used for
conditions where no concentration or dilution series is specified, clicking
“Add ___...” will add the condition directly to the plate with no intervening
options.
7.10.2.4 Adding Multiple Well Items
To sequentially add multiple items to a grouping of wells (i.e. cells, growth
conditions, compounds), select the plate map icon, second-to-the-right on
the top menu, that reads “Keep Current Selection After Adding to Wells”.
Normally after adding an item to a well or group of wells, that grouping will
automatically be deselected. With the “Keep Selection…” icon activated, the
selected grouping will remain selected after addition of a well item.
7.10.2.5 Deleting/Editing Well Items from Lists
Once the three lists are populated, the User may edit or delete items from
the lists. Editing an item will open the same window as when adding an item
to a list, only the fields in the window will already contain the information for
the selected item. Edit the desired fields (Short Name, Description, Color)
and hit “OK” to save the changes. To delete an item from a list, simply click
on the item, hit “Delete,” and “OK”.
NOTE: This action does not delete any instances of the item already added to
the plate map.
7.10.2.6 Editing Well Contents
Once a well or group of wells are populated, the User can edit its contents
and appearance. To edit well contents, first select the desired wells by left-
clicking over them, then right-click within the selected region to bring up the
editing window. From here, the User can create a custom description of the
selected well(s), delete items, and change the color scheme of the well(s).
Alternatively, use the “Undo” and “Redo” buttons in the menu bar at the
top-left of the window if a mistake is made while editing a well’s contents. In
addition, text size/font/style can be changed using the font button in the
menu bar.
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