Essen BioScience IncuCyte ZOOM - 9.1 Images and What they Mean

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9.1 Images and What they Mean

PROBLEM

SOLUTION

The edges of the wells seem to be out of

focus at the 4X objective.

Visual edge effects can be eliminated by

increasing the total media volume in

each well. However, this will not

dramatically change the proliferation

data. Sufficient proliferation data can

still be generated with only 100 µL in

each well.

Focus issues caused by air bubbles on the

bottom and top of the well.

Air bubbles must be manually removed

by the user to obtain desired images. Air

bubbles should be removed by modifying

pipetting technique or using a de-bubbler

before placing vessels in the IncuCyte™

ZOOM.

100 µL

250 µL