5.1 IncuCyte™ Control Software Installation using Windows XP ........................................................ 20
5.2 IncuCyte™ Control Software Installation using Windows Vista and Windows 7 .......................... 21
5.3 Connecting .................................................................................................................................... 21
5.3.1 Setting the Date and Time .................................................................................................... 21
5.3.2 Establishing User Accounts ................................................................................................... 22
5.4 Remote Administration of the Controller ..................................................................................... 23
Chapter 6: Calibrating the IncuCyte™ ZOOM System ............................................................................. 24
6.1 System Calibration ........................................................................................................................ 24
6.2 Fluorescence Calibration ............................................................................................................... 26
6.2.1 FLR Calibration Results .......................................................................................................... 27
Chapter 7: Cell Culture Monitoring and Data Analysis ........................................................................... 27
7.1 Introduction .................................................................................................................................. 27
7.2 User Interface ............................................................................................................................... 28
7.3 Scheduling Scans and Loading Vessels .......................................................................................... 29
7.3.1 24-Hour Repeating ................................................................................................................ 29
7.3.2 Scan on Demand ................................................................................................................... 34
7.4 Finding and Viewing Scanned Vessels ........................................................................................... 35
7.4.1 Finding Scanned Vessels ....................................................................................................... 35
7.4.2 Vessel View ........................................................................................................................... 37
7.5 Data Processing ............................................................................................................................. 40
7.5.1 Assay Mode vs. Established Mode ........................................................................................ 40
7.5.2 Analysis Job Utilities .............................................................................................................. 41
7.6 Graphing and Exporting Data ........................................................................................................ 47
7.6.1 Create a Graph ...................................................................................................................... 47
7.6.2 Microplate Graphing ............................................................................................................. 61
7.6.3 Exporting Experimental Data ................................................................................................ 62
7.6.4 Saving and Printing Graphs ................................................................................................... 66
7.7 Exporting Images and Movies ....................................................................................................... 66
7.7.1 Exporting Images Overview .................................................................................................. 66
7.7.2 Exporting Movies Overview .................................................................................................. 72