10-1562 Issue 3-7 March 2014 39
Creating new methods
New methods can be created either manually or using settings suggested by the automatic
programming software (refer to Advanced Operation ā Setting-up a method).
Alternatively, existing methods can copied to a new location using the COPY TO function in
menu bar of the SELECT RUN screen, then optimised for a specific application. This allows
runs to be created for development whilst the original is preserved.
Method optimisation
Experimentation will be required when optimising methods. To establish a benchmark,
perform an evaporation using both SampleGuard probes and monitor the sample drying
process. The collected data will indicate when the evaporation process is complete, for fast
lyophilisation methods, it will also indicate when the monitored sample has frozen and when
the lyophilisation process ends.
Use the data to optimise the method; for example, if it can be proven that a four hour method
actually dries the samples in three and a half hours, reduce the run time.
Several trials using different control regimes can then be tried to see if the run time can be
reduced further. Once a fully functioning process has been optimised, methods using
Elapsed Time for the Heat-Off and End-of-Run options, may employ single SampleGuard
channel temperature control. Genevac are able to provide remote assistance by analysing
evaporation data from a log file.
Lyophilisation
When performing lyophilisation, fill all positions of the sample holders as samples in partially
filled holders may not freeze. It normally requires several trials to establish a robust
lyophilisation method, your local Genevac representative will be happy to assist with this.
To Next Page
Loading...
