Genevac HT-24 - Application-Specific Method Examples

Genevac HT-24
User Manual
42 10-1562 Issue 3-7 March 2014
Method examples
The following examples show suggested settings for some common applications.
Example 1. Drying HPLC Fractions
Method consists of three runs linked together to form a multi stage run, with settings as
follows:
Stage 1
Remove Organic
Phase
Stage 2
Remove most of the
Aqueous Phase
Stage 3
Remove Final
“Stubborn” Solvents
Run No.
1
2
3
Run Data Protection
N
N
N
Next Run Number (0 for none)
2
3
0
Run Name
Fractions
Fractions 2
Fractions 3
Sample Holder Type
Your Holder
Your Holder
Your Holder
Sample Volume (ml)
Your Volume
Your Volume
Your Volume
Solvent
MeOH & H
2
0
MeOH & H
2
0
MeOH & H
2
0
SampleGuard Control Temp (°C)
40
40
40
SampleGuard Control Channel
1
1
1
CoolHeat Enable Pressure (mbar)
50
50
50
Chamber Temperature control (°c)
preheat to 25
preheat to 30
preheat to 30
Rotor Speed
High
High
Low
Heat-Off Option
Elapsed Time
Heat Flow
Elapsed Time
Heat-Off Elapsed Time (HH:MM)
00:30 to 01:00 *
-
01:00 - 03:00
End-of-Run Option
Elapsed Time
-
Elapsed Time
End-of-Run Elapsed Time (MM:MM)
00:30 to 01:00
-
01:00 - 03:00
Auto Defrost & Drain
Y
N
Y
Pressure Control Regime
Variable Dri-Pure
Controlled Pressure
Full Vacuum
Dri-Pure Start Pressure (mbar)
175
-
-
Dri-Pure End Pressure (mbar)
40
-
-
Dri-Pure Ramp Time (MM)_
20
-
-
Final Control Pressure (mbar)
45
8
* Set Heat-off Elapsed Time to the same as End-of-Run Elapsed time.