Genevac HT-24 - Page 45

User Manual
Genevac HT-24
10-1562 Issue 3-7 March 2014 43
Example 2. Fast Lyophilisation of HPLC Fractions
Method consists of three runs linked together to form a multi stage run, with settings as
follows:
Stage 1
Ramp & Remove
Organic Phase
Stage 2
Freeze & Primary
Drying
Stage 3
Secondary
Drying
Run No.
1
2
3
Run Data Protection
N
N
N
Next Run Number (0 for none)
2
3
0
Run Name
Fractions Lyo
Fractions Lyo 2
Fractions Lyo 3
Sample Holder Type
Your Holder
Your Holder
Your Holder
Sample Volume (ml)
Your Volume
Your Volume
Your Volume
Solvent
MeOH & H
2
0
MeOH & H
2
0
MeOH & H
2
0
SampleGuard Control Temp (°C)
40
0
40
SampleGuard Control Channel
1
1
1
CoolHeat Enable Pressure (mbar)
50
50
50
Chamber Temperature control (°c)
preheat to 20
preheat to 0
preheat to 30
Rotor Speed
High
High
Low
Heat-Off Option
Elapsed Time
Elapsed Time
Elapsed Time
Heat-Off Elapsed Time (HH:MM)
00:45
0:00
05:00
End-of-Run Option
Elapsed Time
Elapsed Time
Elapsed Time
End-of-Run Elapsed Time (MM:MM)
00:45
03:00
15:00
Auto Defrost & Drain
N
N
Y
Pressure Control Regime
Variable Dri-Pure
Full Vacuum
Full Vacuum
Dri-Pure Start Pressure (mbar)
120
-
-
Dri-Pure End Pressure (mbar)
1
-
-
Dri-Pure Ramp Time (MM)_
45
-
-
Final Control Pressure (mbar)
0
-
Note: There are a number of variables that can significantly affect the timings of
lyophilisation methods. For assistance in optimising fast lyophilisation methods, contact
applications@genevac.co.uk