Nitrogen, Total Inorganic
Nitrogen, Total Inorganic
Page 2 of 6
NitrogenTI_TNT_Other_TTR_Eng_Ody.fm
5. Pour the contents of
one Total Inorganic
Nitrogen Reductant
ampule into the vial
containing the sample.
Pour the contents of
another Total Inorganic
Nitrogen Reductant
ampule into the vial
containing the blank.
A black precipitate will
form immediately.
6. Cap the vials. Shake
gently for 30 seconds to
mix the reagents. Allow
the vials to sit for at least
one minute.
The precipitate should
remain black after
shaking. Excessive
shaking will cause the
precipitate to turn white
and cause low results.
7. Place the vials in a
centrifuge.
(If you do not have a
centrifuge, wait at least
30 minutes for the solids
to settle at the bottom of
the vial. Proceed to step 9.
8. Touch the timer icon.
Touch
OK.
A three-minute timer will
begin.
Centrifuge the vials for
three minutes.
9. Using a pipet, add
2 mL of centrifuged
sample to an AmVer™
Diluent Reagent Test ‘N
Tube™ for Low Range
Ammonia Nitrogen.
Add 2 mL of centrifuged
blank to another AmVer™
Dilutent Reagent Test ‘N
Tube™ for Low Range
Ammonia Nitrogen.
Pipet carefully to avoid
disturbing the sediment.
10. Using a funnel, add
the contents of one
Ammonia Salicylate
Reagent Powder Pillow
(for 5-mL samples) to
each vial.
11. Using a funnel, add
the contents of one
Ammonia Cyanurate
Reagent Powder Pillow
(for 5-mL samples) to
each vial.
12. Cap the vials tightly
and shake thoroughly to
dissolve the powder.