Hach DR2400 - Page 585

PCB (Polychlorinated Biphenyls)
PCB (Polychlorinated Biphenyls)
PCB_None_Other_IMM_Eng_Ody.fm Page 5 of 10
Color Development
Note: Timing is critical; follow instructions carefully
12. With the cuvettes still
held snugly in the rack,
pipet 0.5 mL of Color
Developing Solution into
each Antibody Cuvette.
Note: Use a new pipette tip for
each cuvette.
13. Touch the timer icon.
Enter 5 minutes. Touch
OK.
A 5-minute reaction
period will begin. Mix
following the instructions
in Using the 1-cm
MicroCuvette Rack.
14. After 2.5 minutes,
mix the contents of the
rack a second time for a
period of 30 seconds
using the same
technique.
15. At the end of the
5-minute reaction period,
pipette 0.5 mL of Stop
Solution into each cuvette
in the same order as the
Color Developing
Solution was added in
step 12.
Slide the rack for
20 seconds using the
technique described in
Using the 1-cm
MicroCuvette Rack on
page 7.
Note: Blue solutions will turn
yellow with the addition of the
Stop Solution.
Note: The same pipette tip can
be used repeatedly for this
step.
HRS MIN SECHRS MIN SEC
HRS MIN SECHRS MIN SEC
HRS MIN SECHRS MIN SEC