DR/2400
Phosphorus, Reactive (Orthophosphate)
PhosphorusReac_TNT_High_MOL_Eng_Ody.fm Page 1 of 4
• Reagent blanks for each lot of reagents may be used more than once. At room temperature, the reagent blank is stable for up to
three weeks.
• The seven-minute reaction time in step 4 is for samples at 23 °C. For samples at 13 °C, wait 15 minutes. For samples at 33 °C,
wait two minutes.
• Wipe the outside of sample cells before each insertion into the instrument cell holder. Use a damp towel followed by a dry one to
remove fingerprints or other marks.
• Final samples will contain molybdenum. Also, final samples will have a pH less than 2 and are considered corrosive (D002) by the
Federal RCRA. Consult the Material Data Safety Data Sheet for information specific to the reagents used.
Phosphorus, Reactive
(Orthophosphate)
Method 8114
Molybdovanadate Method*
*Adapted from Standard Methods for the Examination of Water and Wastewater
Test ‘N Tube™ Vials
HR (1.0 to 100.0 mg/L PO
4
3–
)
Scope and Application: For water and wastewater
1. Touch
Hach Programs.
Select program
540 P React. HR TNT.
Touch
Start.
2. Use a TenSette
®
Pipet
to add 5.0 mL of
deionized water to a
Reactive High Range
Phosphorus Test ‘N Tube
Vial (this is the blank).
Cap and invert to mix.
3. Use a TenSette Pipet
to add 5.0 mL of sample
to a Reactive High Range
Phosphorus Test ‘N Tube
Vial (this is the sample).
Cap and invert to mix.
4. Touch the timer icon.
Touch
OK.
A 3-minute reaction
period will begin.
Read the sample within
two minutes after the
timer beeps.
Tips and Techniques
Test ‘N Tube Method 8114
Hach Programs