Tannin and Lignin
Tannin and Lignin
TanninLig_None_Other_TYR_Eng_Ody.fm Page 3 of 4
Accuracy Check
Standard Solution Method
Prepare a 200-mg/L tannic acid standard solution as follows:
1. Dissolve 0.200 grams of tannic acid in deionized water and dilute to
1000 mL. Prepare this solution monthly. Further prepare a 6.0-mg/L tannic
acid standard by diluting 15.00 mL of the stock solution to 500 mL with
deionized water. Prepare this standard daily. Perform the tannin and lignin
test on the standard solution as described above.
2. To adjust the calibration curve using the reading obtained with the
6-mg/L standard solution, touch
Options on the current program menu.
Touch
Standard Adjust.
3. Touch
On. Touch Adjust to accept the displayed concentration. If an alternate
concentration is used, touch the number in the box to enter the actual
concentration, then touch
OK. Touch Adjust.
Method Performance
Precision
Standard: 6.00 mg/L tannic acid
See Section 3.4.3 Precision on page 44 for more information, or if the standard
concentration did not fall within the specified range.
Sensitivity
See Section 3.4.5 Sensitivity on page 44 for more information.
Summary of Method
This test measures all hydroxylated aromatic compounds, including tannin,
lignin, phenol, and cresol. This method produces a blue color proportional to the
amount of these compounds present in the sample. The results are reported as
total tannin and lignin and expressed as mg/L tannic acid. Test results are
measured at 700 nm.
Program 95% Confidence Limits of Distribution
720 5.9–6.1 mg/L tannin
Portion of Curve ∆Abs ∆Concentration
Entire range 0.010 0.07 mg/L tannin
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