Toxic ity
Tox ici ty
Toxicity_None_Other_Eng_Ody.fm Page 3 of 6
5. Touch Zero.
The display will show:
0.000 ABS
6. Label a cell “control”.
Open one ToxTrak
Reagent Powder Pillow
and add the contents to
the empty reaction cell.
7. For each sample or
dilution, repeat step 6.
Label each cell.
8. Add 5.0 mL of
deionized water to the
control cell.
Use deionized water that
is free of toxicity or
another water source that
represents baseline
toxicity.
9. Add 5.0 mL of sample
(or dilutions) to each
sample cell.
To determine the
approximate threshold
level of toxicity for a
sample, dilute 1 mL of
sample to 10 mL with
deionized water and run
the test. Continue to make
serial 1/10 dilutions of the
sample until a level is
reached that gives 0%
Inhibition in the final
calculation.
10. Add two drops of
Accelerator Solution to
each cell. Cap and shake
to mix.
Shaking fully oxygenates
the samples and assures
that the oxygen
concentration is not a
factor in determining the
respiration rate.
11. Add 0.5 mL of
inoculum (previously
prepared) to each cell.
Cap and invert to mix.
12. Place the control in
the adapter. Record the
absorbance.
Note: See the Instrument
Manual for more information on
taking a reading.
Zero
C
Repeat
Step 6
C
S
1
S
2
C
S
2
S
1
C
S
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S
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