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HP D300 User Manual

HP D300
74 pages
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HP D300 Digital Dispenser
Operating Manual

Table of Contents

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HP D300 Specifications

General IconGeneral
BrandHP
ModelD300
CategoryDispenser
LanguageEnglish

Summary

1 Product description

1-1 System requirements

Details hardware, software, and environmental conditions required for the HP D300 Digital Dispenser operation.

2 Safety

2-1 General safety rules

Outlines essential safety practices for operating the dispenser, including placement, power, and avoiding moving parts.

2-2 Personal protective equipment

Specifies recommended PPE for fluid handling operations, matching company safety standards.

2-3 Possible inhalation hazard

Addresses potential aerosol generation and recommends using exhaust enclosures for hazardous fluids.

2-4 Pinch hazard

Warns about pinch points between dispenser components and advises safe handling to avoid injury.

2-5 Laser safety

Explains Class 1 laser safety compliance and warnings for the destination plate height sensor.

2-6 Electrical safety

Covers electrical safety certifications, voltage, amperage, and fuse specifications for the dispenser.

2-7 Electrical emissions safety

Details FCC/EMC compliance for emissions and potential radio frequency interference.

2-8 Decontamination prior to servicing

Explains requirements for cleaning and decontamination before shipping the dispenser for service.

2-9 Safe disposal of Dispensehead Cassettes and pipette tips

Provides guidance on proper disposal procedures for used dispensehead cassettes and pipette tips.

2-10 Ergonomic safety

Offers recommendations for workstation setup to minimize ergonomic risks during dispenser operation.

2-11 Additional assistance

Directs users to Tecan Service Help Desk for further support and assistance.

3 Clean the dispenser

4 Get started

4-1 Start up the dispenser

Step-by-step guide to unpacking, connecting, powering on, and installing software for the dispenser.

4-2 Get familiar with the software

Introduces the main operating window, software startup routines, and basic interface elements.

4-3 Load an existing plate configuration

Explains how to load pre-saved plate configurations (.xls files) into the software for dispense operations.

4-4 Get to know the Options dialog box

Overview of the Options dialog box and its various panes for customizing software settings and dispenser behavior.

4-5 Plate dispense procedure

Details the steps involved in executing a plate dispense operation, from initiation to completion.

4-5-2 Scan and load the well plates

Instructions for loading and verifying the destination well plate on the dispenser's plate holder.

4-5-3 Load a Dispensehead Cassette

Guide on how to properly load a new Dispensehead Cassette into the instrument.

4-5-4 Load fluid into the Dispensehead

Explains the process of filling Dispenseheads with fluids using the Load Fluid dialog box.

4-6 View the report

Describes how to access and view dispense operation reports generated by the software.

4-7 Turn off the HP D300 Digital Dispenser

Provides instructions for safely shutting down the dispenser and closing the software.

5 Create plate configurations

5-1 Create the fluids table

Guides users on defining fluids, source plates, wells, and concentrations in the software's fluids table.

5-2 Edit the Fluids List

Details methods for editing, copying, pasting, and managing fluids within the software's Fluids List.

5-3 Edit the well plate

Explains how to add fluids to wells, set titrations, and manage the well plate image.

5-4 Set concentrations or volumes in wells

Describes how to assign specific concentrations or volumes to selected wells for dispensing.

5-5 Use the Paste Special dialog box

Guides on replicating plate designs and mapping fluids using the Paste Special function for efficiency.

5-6 Normalize

Explains how to program DMSO normalization for wells to ensure consistent assay volumes.

5-7 Randomize

Describes how to randomly reorder wells to minimize systematic plate edge effects on output data.

5-8 Save the plate configuration

Instructions for saving the current plate design as an Excel file for future use and editing.

5-9 Plate Layout Wizard basics

Introduces the Plate Layout Wizard for creating simple and complex plate designs efficiently.

5-9-2 Position NoDispense controls

Details how to position NoDispense fluids (blanks or controls) within the well plate using the Wizard.

5-9-3 Position a single zone titration1

Explains how to position single zone titrations within the well plate layout using the Wizard.

5-10 Plate Layout Wizard advanced functions

Covers advanced plate design configurations using the Wizard for complex assays.

5-10-1 Create multizone titration1 layouts

Guides on creating titrations with unevenly spaced concentrations for specific assay regions.

5-10-2 Create modified range titration1 layouts

Explains how to adjust the dynamic range of titrations by modifying fluid concentrations.

5-10-3 Create titration2 layouts: two fluids per well

Details how to create layouts with two fluids per well for drug-drug interaction studies.

6 Troubleshooting and error messages

6-1 Troubleshooting

Provides general troubleshooting steps for software, computer, stage, and fuse issues.

6-2 Error messages

Lists common error messages and their recommended actions for resolution.

Appendix A HP D300 Digital Dispenser software basics

Global view

Describes the global view of the plate showing all dispensed fluids, regardless of editing status.

Plate Layout Wizard

Explains the purpose and functionality of the Plate Layout Wizard for creating plate configurations.

Working with the software

Covers software interaction basics like closing windows, using buttons, and shortcut keys.

Appendix B Glossary

Appendix C Quick Start Guide

Appendix D Revision History