HP D300 Digital Dispenser
Contents
1 Product description
1-1 System requirements ..................................................................................................................................... 6
1-1-1 Hardware ............................................................................................................................................... 6
1-1-2 Software ................................................................................................................................................ 6
1-1-3 Environmental operating conditions ...................................................................................................... 6
1-1-4 Shipping and storage conditions ........................................................................................................... 6
2 Safety
2-1 General safety rules ....................................................................................................................................... 7
2-2 Personal protective equipment ....................................................................................................................... 7
2-3 Possible inhalation hazard ............................................................................................................................. 7
2-4 Pinch hazard .................................................................................................................................................. 7
2-5 Laser safety .................................................................................................................................................... 8
2-6 Electrical safety .............................................................................................................................................. 8
2-7 Electrical emissions safety ............................................................................................................................. 8
2-8 Decontamination prior to servicing ................................................................................................................. 9
2-9 Safe disposal of Dispensehead Cassettes and pipette tips ........................................................................... 9
2-10 Ergonomic safety ......................................................................................................................................... 9
2-11 Additional assistance ................................................................................................................................... 9
3 Clean the dispenser
4 Get started
4-1 Start up the dispenser .................................................................................................................................. 11
4-2 Get familiar with the software ....................................................................................................................... 12
4-3 Load an existing plate configuration ............................................................................................................. 14
4-4 Get to know the Options dialog box ............................................................................................................. 16
4-4-1 Options: General ................................................................................................................................. 17
4-4-2 Options: Edit: Units .............................................................................................................................. 17
4-4-3 Options: Edit: Fluid Defaults ................................................................................................................ 18
4-4-4 Options: Edit: Assay Defaults .............................................................................................................. 19
4-4-5 Options: Plate Jitter ............................................................................................................................. 19
4-4-6 Options: Report ................................................................................................................................... 20
4-5 Plate dispense procedure ............................................................................................................................ 21
4-5-1 Initiate plate dispense .......................................................................................................................... 21
4-5-2 Scan and load the well plates .............................................................................................................. 22
4-5-3 Load a Dispensehead Cassette .......................................................................................................... 22
4-5-4 Load fluid into the Dispensehead ........................................................................................................ 23
4-6 View the report ............................................................................................................................................. 27
4-7 Turn off the HP D300 Digital Dispenser ....................................................................................................... 30
5 Create plate configurations
5-1 Create the fluids table .................................................................................................................................. 31
5-2 Edit the Fluids List ........................................................................................................................................ 32
5-3 Edit the well plate ......................................................................................................................................... 33
5-4 Set concentrations or volumes in wells ........................................................................................................ 35
5-5 Use the Paste Special dialog box ................................................................................................................ 38
5-6 Normalize ..................................................................................................................................................... 39
5-7 Randomize ................................................................................................................................................... 41
5-8 Save the plate configuration ......................................................................................................................... 42
5-9 Plate Layout Wizard basics .......................................................................................................................... 43
5-9-1 Create single zone titration1layouts .................................................................................................... 43
5-9-2 Position NoDispense controls .............................................................................................................. 47
5-10 Plate Layout Wizard advanced functions ................................................................................................... 49
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