14
On the contrary, knowing the volume of your dose (for example 9 mL) and the maximum precision you
want (for example 2%) - follow the dots in black -, a tubing with an inner diameter of 3,2 mm is sufficient
because its curve goes underneath the intersections (lines in dots).
Note : the lines in black dots are engraved on the side of the FlexiPump
®
, but not the red ones.
9 mL = typical volume to dilute a 1mL sample at 1/10
th
.
18 mL = typical volume to fill a 90 mm Petri dish with agar.
225 mL = typical volume to dilute a 25 g sample at 1/10
th
.
With FlexiPump
®
Pro, you get a better precision than with the FlexiPump
®
thanks to the 2 tubings
associated with the 2 pumps out of phase.
The typical precision values obtained, with a tubing with an inner diameter of 3,2 mm are the following :
FlexiPump
®
:
9 mL : 1,8 % - 18 mL : 1 % - 225 mL : 0,5 %
FlexiPump
®
Pro
:
9 mL : 0,9 % - 18 mL : 0,5 % - 225 mL : 0,4 %
3.5.2 Programs and calibration
A calibration is necessary for programs distributing doses with a fixed volume. The calibration enables
the FlexiPump
®
to measure the real flow with the conditions of the program, to memorize it and to take it
into account at each dose delivered. The flow rate being known, the FlexiPump
®
makes the number of
rotations necessary for each dose.
The flow delivered by the pump will indeed depend on the rotation speed; the given rotation speed of a
given pump, will depend upon the diameter of the tubing and the viscosity of the liquid. The flow directly
proportionnally depends on the rotation speed but this proportionnality depends on the tubing diameter
and the viscosity of the liquid.
Moreover, with a same tubing and liquid, the medium flow can vary according to the volume of a dose,
especially when going from a few microliters to several liters.
As each program has different parameters: volume of a dose, tubing diameter and liquid viscosity; a
calibration is necessary for each program.
3.5.3 Programs: user manual
More information about the use of programs are given at the chapter 3.7 Programs.
3.6 General settings
The access to programing functions in the general settings is made by the choice of the
“CONFIGURATION” option in the main menu or by pressing the “change settings” button on the
top right of the rotating button.
Here are the options appearing in the settings menu in the general settings:
CONFIGURATION
FLOW DIRECTION:
ANTI-DROP: yes
SOUND: yes
CONTRAST
DATE / TIME
NAME: .........
LANGUAGE:english
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