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Merck Millipore ReadyStream - Page 20

Merck Millipore ReadyStream
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20
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The user is asked to conrm lling to begin by pressing
[+]. This will start the water pump and turn on the
heaters to reconstitute the media with preheated water.
While the media bag is lled, the “Fill” LED is blinking
green and the remaining lling volume is displayed.
The media bag must be softly agitated by gently
pressing all over the bag surface during the lling step.
If needed, agitate the back of the bag to detach the
media stuck on the lm.
Do not keep your nger on the valve actuator
while it is moving.
When pumping deionized water from a
container, ensure enough water is available to
cover the lling step.
Do not close the peristaltic pump head until
the lling process is over. To prevent internal
overpressure the system will stop dispensing,
report an error and the media bag will need to
be discarded.
Start the bag agitation during the lling step.
In the unlikely event of an issue during media bag
lling (e.g. leakage), press [CANCEL] to stop the
water pump safely. The “Filter, “Bag” and “Prog”
LEDs will ash red.
Filling cannot be resumed, and therefore the media
bag must be discarded.
When the bag is lled, the “Fill” LED turns to solid
green.
The “Bag” LED is blinking green and the user is
prompted to agitate the granulated media with the
added water.
Additionnal continuous soft bag agitation is required
during a maximum of 2 min to speed up the
dissolution of the granules.
Use the side switch (Figure 2, R3) at the front, right
side, of the instrument to turn on the panel of LEDs
to illuminate the media bag. Check that no clumps
of media or a large quantity of undissolved granules
are remaining, otherwise, please continue to gently
agitate the bag.
The turbid concentrated media will become clear
during the remaining rest time indicated on the
display.
When the time has elapsed, the “Bag” LED will blink
orange and the user is asked to conrm that the
media is fully dissolved.
Carefully inspect the bag for undissolved media.
Continue to softly agitate the bag if required until
there is no granulated media left, then press [+].

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