Merck Millipore ReadyStream - Calibration of the peristaltic pump

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Do not conrm dissolution if any undissolved
media remains, as this could lead to media of
a wrong concentration being dispensed.
The “Filter”, “Prime”, “Bag”, and ”Fill” LEDs are all solid
green at this stage to indicate that the lter is primed and
the media bag stock is properly reconstituted.
The peristaltic pump head has to be closed at this stage,
after bag reconstitution is completed. Apply little tension
on the silicone tubing to make sure the tube is fully
horizontal between the stopcock actuator and the pump
before closing the head.
Ensure the tubing is not pinched in the closed
pump head.
If the peristaltic pump head is not closed, the “Filter”,
“Bag”, and “Prog” LEDs will all blink orange and the
user is prompted to close the pump head.
Calibration of the peristaltic pump
Following conrmation that the media bag stock is fully
reconstituted, the instrument will automatically move on
to the peristaltic pump calibration step once the pump
head is closed.
To ensure precision in media dispensing, the peristaltic
pump must be calibrated each time a new bag is installed
on the instrument, or each time the pump head is
accidentally opened.
Calibration is performed by weighing a dened quantity of
water dispensed by the instrument.
For precise calibration, the dispensing outlet tubing must
be ushed to be free of any air bubbles. The “Water” LED
will be blinking orange and the user must press [WATER]
to start the water and media pumps lling the dispensing
tubing with water.
Make sure to have a container/bag on
the dispensing unit to collect the media
dispensed, and that the orange cap is
removed.
During priming, the “Water” LED will blink green.
When the tubing is properly ushed, press [WATER]
once more to stop the pump.
Make sure no air bubbles are visible in the
tubing before stopping the pump.
The user is then prompted to tare a container on a
balance (not provided), and to position the container on
the dispensing unit (container can also be a bag placed
in the bag holder).
The “Media” LED will be blinking orange.
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Alternating every
3 sec with
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