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Nicoya OpenSPR User Manual

Nicoya OpenSPR
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NICOYA LIFESCIENCES
1-CHANNEL OPERATION MANUAL V4.1.01
Released: January 18, 2018 (V4.1.01)
support@nicoyalife.com

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Nicoya OpenSPR Specifications

General IconGeneral
TypeSurface Plasmon Resonance (SPR) Instrument
Data AcquisitionReal-time
ConnectivityUSB
Operating System CompatibilityWindows, MacOS
Detection MethodLabel-free
Measurement Range1 picomolar to 1 micromolar
SoftwareOpenSPR Software
ThroughputSingle channel
DetectionRefractive Index changes near the sensor surface
ApplicationsProtein-protein interaction, antibody characterization, small molecule binding, DNA/RNA interactions, kinetics, affinity, concentration, biosensing
Detection Range1 picomolar to 1 micromolar
Dimensions38 x 28 x 25 cm

Summary

GETTING STARTED

1.1 Initial Equipment Setup

Setup of the OpenSPR instrument and initial requirements.

1.2 OpenSPR Software Setup

Requirements and installation steps for OpenSPR software.

1.3 TraceDrawer Software Setup

Setup and license activation for TraceDrawer software.

STANDARD OPERATING PROCEDURES

2.1 Preparation for your Experiment

Required items and fluidic/optical setup for experiments.

2.2 Beginning a Test

Steps to initiate a new test and connect the instrument.

2.3 Instrument Setup

Configuring running buffers, priming fluidics, and optical references.

2.4 Sensor Setup

Installing, loading, and preparing the sensor chip.

2.5 Running an Experiment

Steps for naming tests, data acquisition, buffer changes, and flow rates.

2.6 Shutdown Procedures

Procedures for ending tests, standby mode, and instrument shutdown.

2.7 Data Output Files

Overview of generated data files and their formats.

MAINTENANCE AND CLEANING

3.1 Preparation of Reagents

Importance of filtered reagents and inspection for particulates.

3.2 Standby Mode

Procedures for placing the instrument into standby mode.

3.3 Instrument Shutdown

Steps for performing a full instrument shutdown.

3.4 Recommended Cleaning

Regular and advanced cleaning procedures to prevent contamination.

SYSTEM DETAILS

4.1 OpenSPR Hardware

Overview of the instrument's external and internal hardware components.

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