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OpenSPR-XT
Nicoya OpenSPR-XT User Manual
82 pages
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NICO
Y
A LIFESCI
ENCES
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V1
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0
Last Updated: April
14, 201
6 (V1
.0
)
support@nicoyalife.c
om
This device complies with
Part 15 of the FCC Rules. Oper
ation is subject to th
e followi
ng two
conditions: (1) this devi
ce may not cause harmful inter
ference, and (2) this device must accept any
interference received, incl
uding interference th
at may cause undesired op
eration. This
device wa
s
manufactured in Can
ada.
2
Table of Contents
Default Chapter
2
Table of Contents
2
Safety and Preparation for Use
4
Operating Conditions
6
Specifications
8
Chapter 1: Getting Started
10
Initial Equipment Setup
10
Before any Steps Are Taken
10
Standard Equipment Supplies
10
Unpacking of Openspr-XT™ Instrument
11
Setting up Fluidic Connections
12
Turning on the Openspr-XT TM
17
Overview of Openspr-XT™ Instrument Components
20
Chapter 2: Operation Guidelines
25
Required Items
25
Experimental Procedure
25
Starting the System
25
Instrument Priming and Obtaining References
26
Loading a New Sensor Chip
29
Bubble Removal & Cooling
32
Sample & Timeline Setup
37
Shut down Procedure
44
Chapter 3: Data Analysis Using Tracedrawer
46
Data Output
46
Tracedrawer Analysis
47
Import Data into Tracedrawer
47
Data Modifications
49
Kinetics Evaluation
54
Additional Resources
58
Chapter 4: Openspr-Xt Tm System Details
59
Hardware
59
Openspr Unit
59
XT Autosampler Unit
62
Software
67
Adjusting the Response Graph
67
Chapter 5: Faq & Troubleshooting
69
Frequently Asked Questions
69
Troubleshooting
69
Chapter 6: Cleaning and Maintenance
72
Start-Up and Diagnostic Routines
72
Instrument Maintenance and Cleaning
73
Sensor Chip Cleaning
74
Installing a New Flow Cell
75
Changing the Sample Loop
75
Chapter 7: Theory
78
Localized Surface Plasmon Resonance
78
Binding Kinetics
79
Steady State Affinity Experiments
80
Kinetic Affinity Experiments
81
Recommended Reading
81
References
82
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Nicoya OpenSPR-XT Specifications
General
Type
Surface Plasmon Resonance (SPR) Instrument
Connectivity
USB
Software
TraceDrawer
Detection
Label-free
Data Analysis Software
TraceDrawer
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