54
3.2.3 Kinetics Evaluation
Generally, a sugar test injection (25 mg/ml in the running buffer) is performed under the same
experimental conditions to help determine the dispersion characteristic of sample injections. For
example, at 20µl/min pump speed, the actual association period is around 200s as shown below [Figure
3.16].
Figure 3.16 - Resulting sensor response after a sugar test injection. The start of concentration change is indicated by the red dot
and the end of concentration change is indicated by the blue dot. The association period is the amount of time between the two
dots.
10. Click New Evaluation to open an add evaluation item window. From the Evaluation type drop
down list, choose Kinetics evaluation. Select the desired overlay from the Data box and click OK
[Figure 3.17].
Figure 3.17 - To begin kinetics evaluation, select New Evaluation (left). Under Evaluation type, select Kinetics evaluation and the
desired overaly to analyze (right).
To Next Page
Loading...
