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This procedure provides the required 10 minutes between each injection necessary for proper
cleaning of the instrument.
5. Begin the test procedure after water has been flowing through the system for at least 15
minutes.
6.3 Sensor Chip Cleaning
Organic contaminants can accumulate on the sensor surface in storage over time that may affect the
sensitivity of the sensor. Removal of the contaminants before testing will ensure optimal instrument
performance. Outlined below are 4 methods to remove contaminants dependent on the facilities
offered in user’s laboratory. Only use these cleaning methods when working with uncoated, plain
gold Sensor Chips. Do not use with NTA, Streptavidin, or Amine Sensor Chips.
Method 1:
The most effective cleaning procedure utilizes piranha solution. It is not recommended that this be
conducted by anyone without previous experience using piranha as it is extremely hazardous. All
safety protocols must be observed when using this procedure. First, the chip should be placed in an
isopropanol bath for 5 minutes and dried with nitrogen. Under a fumehood and wearing the
appropriate PPE, a small amount of piranha (200 µL) can be pipetted onto the sensor and left for 10
minutes. The piranha should be safely removed from the sensor by gently squirting distilled water over
the sensor and catching the liquid in a designated acid waste beaker. Do not directly squirt the sensor
as this may damage the surface. Finally the chip should be immersed in a pure ethanol bath for at least
30 minutes and dried with nitrogen before use.
Method 2:
For customers that do not wish to use piranha, neat sulfuric acid can be used instead without the
addition of peroxide. Place the sensor in an isopropanol bath for 5 minutes and dry with nitrogen.
Place the dried sensor into a petri dish still under a fume hood and wearing appropriate PPE, pipette
a small amount of sulfuric acid onto the sensor and leave for 10 minutes. Gently squirt deionized water
over the chip and carefully remove the chip with tweezers. Do not directly squirt the sensor as this
may damage the surface. Immerse the sensor in a pure ethanol bath for at least 30 minutes and dry
with nitrogen before use.
Method 3:
The third cleaning procedure utilizes UV-ozone treatment. A Sensor Chip should be rinsed with
deionized water, ethanol, acetone and isopropanol, dried with nitrogen then placed under UV-ozone
treatment for 15 minutes. The chip should be rinsed again using the four solvents and dried with
nitrogen before use. The procedure can be repeated if necessary.
Method 4:
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