Nicoya OpenSPR-XT - Default Chapter; Table of Contents

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Table of Contents
Table of Contents .................................................................................................................................................. 2
SAFETY AND PREPARATION FOR USE ..................................................................................................................... 4
OPERATING CONDITIONS ................................................................................................................................................. 6
SPECIFICATIONS ............................................................................................................................................................. 8
CHAPTER 1: GETTING STARTED ...................................................................................................................... 10
1.1 INITIAL EQUIPMENT SETUP ................................................................................................................................ 10
1.1.1 Before any Steps are Taken.................................................................................................................... 10
1.1.2 Standard Equipment Supplies ................................................................................................................ 10
1.1.3 Unpacking of OpenSPR-XT™ Instrument ................................................................................................ 11
1.1.4 Setting up Fluidic Connections ............................................................................................................... 12
1.1.5 Turning on the OpenSPR-XT
TM
............................................................................................................... 17
1.2 OVERVIEW OF OPENSPR-XT™ INSTRUMENT COMPONENTS ................................................................................... 20
CHAPTER 2: OPERATION GUIDELINES ............................................................................................................. 25
2.1 REQUIRED ITEMS ............................................................................................................................................. 25
2.2 EXPERIMENTAL PROCEDURE .............................................................................................................................. 25
2.2.1 Starting the System ................................................................................................................................ 25
2.2.2 Instrument Priming and Obtaining References ...................................................................................... 26
2.2.3 Loading a New Sensor Chip .................................................................................................................... 29
2.2.4 Bubble Removal & Cooling ..................................................................................................................... 32
2.2.5 Sample & Timeline setup ....................................................................................................................... 37
2.2.6 Shut Down Procedure ............................................................................................................................ 44
CHAPTER 3: DATA ANALYSIS USING TRACEDRAWER ...................................................................................... 46
3.1 DATA OUTPUT ................................................................................................................................................ 46
3.2 TRACEDRAWER ANALYSIS ................................................................................................................................. 47
3.2.1 Import data into TraceDrawer ............................................................................................................... 47
3.2.2 Data modifications ................................................................................................................................. 49
3.2.3 Kinetics Evaluation ................................................................................................................................. 54
3.2.4 Additional Resources .............................................................................................................................. 58
CHAPTER 4: OPENSPR-XT
TM
SYSTEM DETAILS ................................................................................................. 59
4.1 HARDWARE .................................................................................................................................................... 59
4.1.1 OpenSPR Unit ......................................................................................................................................... 59
4.1.2 XT Autosampler Unit .............................................................................................................................. 62
4.2 SOFTWARE ..................................................................................................................................................... 67
4.2.1 Adjusting the Response Graph ............................................................................................................... 67
CHAPTER 5: FAQ & TROUBLESHOOTING......................................................................................................... 69
5.1 FREQUENTLY ASKED QUESTIONS ........................................................................................................................ 69
5.2 TROUBLESHOOTING ......................................................................................................................................... 69
CHAPTER 6: CLEANING AND MAINTENANCE .................................................................................................. 72
6.1 START-UP AND DIAGNOSTIC ROUTINES ................................................................................................................ 72
6.2 INSTRUMENT MAINTENANCE AND CLEANING ........................................................................................................ 73