Nicoya OpenSPR-XT - Chapter 2: Operation Guidelines; Required Items; Experimental Procedure

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Chapter 2: Operation Guidelines
2.1 Required Items
 Computer with installed OpenSPR-XT™ software
 Degassed running buffer
 80% isopropanol wash solution
 OpenSPR™ Flow Cell
 OpenSPR™ Sensor Chip
 96-well microplate
 Waste containers
 Tweezers
 Nitrile or Latex Gloves
2.2 Experimental Procedure
The following section outlines the general experimental procedure guiding you through how to best
operate the OpenSPR-XT™ hardware and software.
Note: Running buffers should be degassed and filtered prior to use with a 0.2 µm filter. Air bubbles and
precipitates can seriously affect results if they form inside the Flow Cell during an experiment. Buffer
should be made fresh each day if possible and allowed to equilibrate to room temperature.
2.2.1 Starting the System
1. Ensure the wash bottle inside the XT autosampler is filled with a solution of 80% isopropanol
in water, and there is sufficient running buffer present in the running buffer bottle to last the
duration of the test.
2. Ensure the Sample Compartment Cover is installed on the XT autosampler unit and the Sample
Compartment Door is closed.
3. Switch ON the OpenSPR™ power switch located at the back of the instrument [Figure 1.14].
The blue LED on the front of the instrument should turn on [Figure 1.16].
4. Switch ON the XT autosampler power switch located at the back of the instrument [Figure
1.15]. The green LED on the front of the instrument should turn on [Figure 1.16].
5. Open the OpenSPR-XT™ program on the computer by double clicking on the program
shortcut. The OpenSPR-XT™ Home Screen will open [Figure 2.1].