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OpenSPR 1-Channel Operation Manual V4.1.01
Table of Contents
SAFETY AND PREPARATION FOR USE ............................................................................................. 4
1 GETTING STARTED .................................................................................................................. 7
1.1 Initial Equipment Setup .................................................................................................... 7
1.1.1 Before any steps are taken ....................................................................................... 7
1.1.2 Standard Equipment Supplies ................................................................................... 7
1.1.3 Setting up the OpenSPR ............................................................................................ 8
1.1.4 Connecting and Turning on the OpenSPR............................................................... 10
1.2 OpenSPR Software Setup ............................................................................................... 11
1.2.1 Minimum Computer Requirements ........................................................................ 11
1.2.2 Installing OpenSPR Software .................................................................................. 11
1.3 TraceDrawer Software Setup ......................................................................................... 12
1.3.1 Installing TraceDrawer ............................................................................................ 12
1.3.2 TraceDrawer License Activation ............................................................................. 12
2 STANDARD OPERATING PROCEDURES ................................................................................. 14
2.1 Preparation for your Experiment ................................................................................... 14
2.1.1 Required Items ........................................................................................................ 14
2.1.2 Fluidic Setup ............................................................................................................ 14
2.1.3 Optical Setup ........................................................................................................... 16
2.2 Beginning a Test ............................................................................................................. 17
2.3 Instrument Setup ........................................................................................................... 18
2.3.1 Naming your Running Buffers ................................................................................. 19
2.3.2 Step 1: Fluidic Priming ............................................................................................ 20
2.3.3 Step 2: Optical References ...................................................................................... 23
2.4 Sensor Setup ................................................................................................................... 27
2.4.1 Step 3: Install Sensor Chip ...................................................................................... 27
2.4.2 Step 4: Bubble Removal and Prevention ................................................................ 33
2.5 Running an Experiment .................................................................................................. 35
2.5.1 Test Name ............................................................................................................... 35
2.5.2 Experiment Data Interface and Data Acquisition ................................................... 35
2.5.3 Changing the Running Buffer .................................................................................. 37
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