Nicoya OpenSPR - STANDARD OPERATING PROCEDURES; 2.1 Preparation for your Experiment

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OpenSPR 1-Channel Operation Manual V4.1.01
2 STANDARD OPERATING PROCEDURES
2.1 Preparation for your Experiment
2.1.1 Required Items
The following materials are required to run a test on the OpenSPR:
• OpenSPR instrument with corresponding power and USB cables
• Computer with installed OpenSPR software
• 4x 250 mL glass buffer bottles (or equivalent)
• Tweezers
• Lint-fee wipes
• Syringes with blunt-end tips installed
• Blank Chip
• OpenSPR Sensor Chip *see optical setup considerations in section below
• Deionized (DI) water, filtered (0.2 µm filter)
• Running buffer, filtered (0.2 µm filter) and degassed
• Solution of 80% isopropanol (v/v in water)
• Relevant Personal Protective Equipment
2.1.2 Fluidic Setup
Prior to starting your experiment, the fluidic setup must be in place for proper function of the
OpenSPR. This includes having the Waste Bottle, DI Water Bottle, and Buffer Bottles filled to the
proper levels with tubes installed correctly. Details for each bottle and tubing setup are provided
below.
Waste Bottle: 250 mL square glass bottle (or equivalent) that does not contain the tube fitting.
• Ensure the Waste Bottle is empty and labelled appropriately as waste.
• Secure the cap onto the bottle.
• Insert all 3 outlet tubes into the holes located on the top of the waste bottle. The outlet
tubes are the bundle of 3 protruding from the very back of the bottle tray.
• Place the Waste Bottle containing the 3 outlet tubes at the back of the Bottle tray, as
shown in [Figure 2.1].