Nicoya OpenSPR - Page 15

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OpenSPR 1-Channel Operation Manual V4.1.01
Figure 2.1 Empty waste bottle installed in the OpenSPR containing 3 outlet tubes.
DI Water Bottle: 250 mL square glass bottle containing a tube fitting
• Fill this bottle with 250 mL of filtered (0.2 µm filter) deionized water.
• Secure the cap onto the bottle.
• Loosen the fitting, and place the 3
rd
inlet tube into the bottle, such that the tube is just
touching the bottom of the bottle.
• Gently tighten the fitting on the tube such that it is secured into place.
• Place the bottle onto the tray of the OpenSPR next to the Waste Bottle [Figure 2.2].
Buffer Bottle 1 and 2: 250 mL square glass bottles containing a tube fitting
• Prepare at least 100-250 mL of each running buffer for your experiment*
• Filter (using a 0.2 µm filter) and degass the running buffers prior to use.
• Fill the appropriate bottles with the running buffer to a maximum level of 250 mL.
• Secure the cap onto the bottle.
• Loosen the fitting and place the 1
st
and or 2
nd
inlet tube into appropriate buffer bottle,
such that the tube is just touching the bottom of the bottle.
• Gently tighten the fitting on the tube such that it is secured into place.
• Place the bottles onto the tray of the OpenSPR [Figure 2.2].