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OpenSPR 1-Channel Operation Manual V4.1.01
2.3.3 Step 2: Optical References
Optical references are important to be able to measure the absorbance of the nanogold sensor
chip to receive an accurate response signal. Once references are obtained for the OpenSPR unit,
they will be valid for multiple tests. The date and time when the previous references were
acquired will be displayed on the main screen [Figure 2.15].
Figure 2.15 Date and time previous references were acquired as well as expected reference for 1-Channel OpenSPR.
It is only recommended to take new references in the below scenarios:
1) The previous references were acquired more than 1 month ago
2) An insufficient absorbance signal is obtained with a new sensor
Optical references will normalize the optics with respect to the ambient lighting in the room (dark
reference) and the LED light source in the OpenSPR (bright reference). After reference are
acquired, the reference graph should look similar to that shown in the figure above [Figure 2.15].
To begin the process to take new references, click the “Acquire New References” button [Figure
2.16] and follow the on-screen prompts.
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