Nikon DIAPHOT 300 - Page 14

13. Switch to the 40 x objective
and re-center the condenser.
Switch to the 40 x objective.
Adjust the image of the field aperture
diaphragm until it is circumscribed with
the viewfield.
If the center of the field aperture
diaphragm image is off the center of the
viewfield (eccentric condition), readjust
with the condenser centering screws.
If the LWD condenser is used, tighten
the condenser refocusing stopper.
14. Perform microscopy with a
desired objective
♦ Switch to a desired objective.
♦ Focus the microscope.
♦ Adjust the field aperture diaphragm
until it is inscribed or circumscribed
with the viewfield.
♦ Adjust the brightness with the ND
filters or the light intensity control dial
on the base. (Maintain the lamp
voltage between 6 and 12. For color
photomicrography, adjust it to
PHOTO. See page 17.)
♦ Stop down the condenser aperture
diaphragm to a range of 70 to 80% of
the objective's numerical aperture.
(See page 22.)
♦ When replacing the specimen, you
can use the refocusing stopper (page
34), condenser refocusing stopper
(page 35), and pillar tilting
mechanism (page 36) for enhanced
efficiency.
Now, the basic microscopic operation is completed.
Improved image quality may be achieved by slightly opening or closing the
condenser aperture diaphragm. (See page 22.)
For phase contrast microscopy, proceed with the following steps 15. to 18.