Nikon DIAPHOT 300 - Page 15

Phase Contrast Microscopy
1 5. Switch to the phase contrast
objective.
Rotate the revolving nosepiece to bring
the phase contrast objective of the
lowest magnification (ex. 4x) into the
optical path. Focus the microscope
with the coaxial coarse/fine focusing
knob. (The phase contrast objective is
identified as it is impressed with the Ph
code. See page 26.)
16. Bring the phase annular
diaphragm into the optical path.
Identify the annular diaphragm that has
the same Ph code as marked on the
objective now in the optical path, and
bring it into the optical path. (If the
system condenser turret unit is used,
rotate the turret to bring the module
having the same Ph code as marked on
the objective into the optical path.)
1 7. Center the annular diaphragm.
Open the field aperture diaphragm to
the full.
If BT1 eyepiece tube is used:
Set the turret assembly to B. While
observing through both eyepieces,
rotate the Bertrand lens focusing
screw to focus on the phase plate
image of the objective and the annular
diaphragm image of the condenser.
If BT2 eyepiece is used:
Remove an eyepiece and insert the centering telescope in place. Holding the
knurled part of the centering telelscope, rotate its eyepiece to focus on both
the phase plate image of the objective and the annular diaphragm image of the
condenser.
Figure 2-1 7