Nikon ECLIPSE E200 MV Series - Place the Specimen Slide on the Stage and Bring the Specimen into the Optical Path; Focus on the Specimen

Chapter 4 Microscopy Procedure (Detailed)
10
5
Place the specimen slide on the sta
g
e and brin
g
the specimen
into the optical path.
(1) Place specimen slide on the stage with
the coverglass facing upward.
(2) Open the claw of the specimen holder
with your finger at the root or the tip tilt
and fix the specimen slide with the claw.
(3) Rotate the stage motion (Y axis and X
axis) knobs to bring the specimen into
the optical path. Illumination light goes
through the specimen.
6
Focus on the specimen.
(1) Rotate the coarse focus knob to raise the
stage to its upper limit.
The coarse focus knob is on the side
without the stage motion knobs.
Do NOT turn the coarse focus knob further
after the stage has reached its upper limit.
This operation will damage the focusing
mechanism.
・ When you raise the stage using the coarse focus knob, take your eyes off the eyepieces and
look at the gap between the upper surface of the specimen and the front of the objective
from the side.
・ Since the working distances of 10x and 4x objectives are long (P.34), these objectives do not
touch the specimen even when the stage is raised to its upper limit provided that the slide
and coverglasses of a standard thickness are used. (The standard thickness for slide is 1.2
mm and that for coverglass is 0.17 mm.)
(3)
(1)
Coverglass
Stage
motion
knob
(
Y axis
)
Stage
motion
knob
(
Xaxis
)
First raise
the stage…
Coarse focus knob
(1)(2)