6
Microscopy Procedure
(Quick Reference)
3
1
Turn on the power switch.
Press the power switch to the “l” position.
2
Lower the condenser slightly down from its upper limit.
Rotate the condenser focus knob to lower the condenser slightly down from its upper limit.
3
Fully open the field aperture and condenser aperture
diaphragms.
(Field diaphragm control is only for microscopes with the field
diaphragm.)
Turn the field diaphragm ring to the left limit to fully open the diaphragm.
Turn the condenser aperture diaphragm lever to the left limit to fully open the diaphragm.
4
Bring the 10× objective into the optical path.
Rotate the revolving nosepiece to bring the 10
×
objective into the optical path.
5
Place the specimen slide on the stage and brin
the specimen
into the optical path.
Rotate the stage motion (Y axis and X axis) knobs to bring the specimen into the optical path.
6
Focus on the specimen. ⇒P10
Turn the focus knobs to focus on the specimen.
7
Adjust the diopter. ⇒P12
Turn the diopter ring on the eyepieces.
8
Adjust the interpupillary distance.
Match the distance between the eyepieces with the distance between your eyes.
9
Focus on the field diaphragm and center its position.
(This adjustment is only for microscopes with the field diaphragm.) ⇒P14
Use the condenser focus knob, field diaphragm ring and field diaphragm centering screws for this
adjustment.
10
Select the objective.
Rotate the revolving nosepiece to the desired objective magnification.
11
Adjust the condenser aperture diaphragm.
Turn the condenser aperture diaphragm lever to the same figure as the magnification of the
objective in the optical path.
12
Focus on the specimen.
Turn the brightness control dial to adjust the brightness of the viewfield and focus on the
specimen with the focus knobs.
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