Nikon ECLIPSE E200 MV Series - Adjust the Field Diaphragm; (This Adjustment Is Only for Microscopes with the Field Diaphragm.); Perform the Microscopy

Chapter 4 Microscopy Procedure (Detailed)
18
13
Adjust the field diaphragm.
(This adjustment is only for microscopes with
the field diaphragm.)
Looking into the eyepieces, turn the field
diaphragm ring until the diaphragm image just
circumscribes the viewfield.
The field diaphragm is used to control the
specimen's illuminated area relative to the
microscope's viewfield. If it is opened to a larger
aperture than necessary, stray light will enter the
viewfield, which may reduce the image contrast.
Every time you change the objective
magnifications, turn the field diaphragm ring until
the diaphragm image just circumscribes the
viewfield.
・ The figures around the field diaphragm show
the approximate positions for field diaphragm
ring corresponding to each objective
magnification when 10x eyepieces are used.
・ For 100x objective, the field diaphragm cannot
be closed enough.
14
Perform the microscopy.
Rotate the stage motion knobs to move the
specimen into the optical path. If the specimen is
out of focus, rotate the focus knobs to focus on the
specimen.
Change the objectives or specimens and repeat the
procedures of steps 10 to 14.
Field
diaphragm
ring
Diaphragm
image
circum-
scribes the
viewfield.
Corresponding
objective
magnification
Stage motion
knob (Y axis)
Stage motion
knob (X axis)