Chapter 4 Microscopy Procedure (Detailed)
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4
Replacing a Specimen Using the Refocusing Mechanism
Try focusing on the specimen with 40x or higher magnification objective. You will find the
specimen is brought very near to the objective.(*1) It will be very difficult to change the
specimen without moving the focus knob. In a case like this, use the refocusing mechanism for
easy specimen replacement.
(1) Use one hand to gently press down
the stage. (*2)
(2) While holding the stage at that position,
change the specimen.
(3) Gradually release the stage so that it
rises slowly. (The stage will return to
the focal position.)
*1： The distance between the front of the objective and the specimen when the specimen is in focus is
called the "working distance" of the objective. For details, see P.34.
*2： When lowering the stage, take great care not to hit the field lens with the condenser and the parts
under the condenser.
Make sure not to
hit the field lens.
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