IV Microscopy (Detailed Procedure)
15
IV
Replacing a Specimen Using the
Refocusing Mechanism
Try focusing on the specimen with 40x or higher magnification objective. You will find the
specimen is brought very near to the objective.
*1
It will be very difficult to change the specimen
without moving the focus knob. In a case like this, use the refocusing mechanism for easy
specimen replacement.
(1) Use one hand to gently press down the
stage.
*2
(2) While holding the stage at that position,
change the specimen.
(3) Gradually release the stage so that it
rises slowly. The stage will return to the
focal position.
*1: The distance between the front of the objective and the specimen when the specimen is in focus is
called the “working distance” of the objective. For details, see p. 42.
*2: When lowering the stage, take great care not to hit the field lens with the condenser and the parts
under the condenser.
Make sure not
to hit the field
lens.
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