Chapter 5 Troubleshooting
5.4 Perfect Focus System
122
Problem Possible cause Countermeasure
No specimen on stage. Set a specimen and focus on it manually.
Objective too far from boundary surface
of specimen.
Rotate focus knobs to move objective
closer to the boundary surface.
Focusing operation is
terminated and the
buzzer sounds, approx.
5 seconds after
pressing the PFS-ON
switch.
Shape or material of specimen not
suitable for PFS.
Use a specimen suited for PFS.
PFS will not function if the cover glass is
too thick or if the refraction index of the
solution is too high (n is close to 1.5).
See “Supported Specimens” on page 83.
Boundary surface not detected within
time limit. (Panel display: ER2)
When using a dry objective, PFS is
turned off for safety if no boundary
surface is detected within 5 seconds.
PFS dichroic mirror not in the optical
path. (Panel display: ER3)
PFS is turned off for safety. Move
dichroic mirror into the optical path and
turn on PFS again.
Nosepiece is placed out of a click-stop
position. (Panel display: ER4)
PFS is turned off for safety. Rotate
nosepiece to a click-stop position and
turn on PFS again.
Limit reached for vertical stage
movement. (Panel display: ER5)
PFS is turned off for safety.
Reflectance of specimen too high.
(Panel display: ER6 or ER7)
Check that a glass-bottomed dish
(No.1S) is used for the specimen.
A long beep sounds
during the perfect focus
operation and the
perfect focus function
ends.
PFS internal error.
(Panel display: ER8 or ER9)
Contact Nikon.
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