Chapter 2 Microscopy
2.5 In-focus Observation with PFS
49
2.5 In-focus Observation with PFS
In-focus observation is an observation method implemented by combining a Ti-E or Ti-E/B microscope with a
PFS Motorized Nosepiece. The system detects the boundary surface between the cover glass and the
aqueous solution of the specimen (for water immersion or oil immersion objectives)
(NOTE 1)
or between the
cover glass and air (for dry objectives)
(NOTE 1)
, and maintains the focus by controlling the focusing
mechanism to track the vertical movement of the boundary surface. This method achieves a constant focal
distance by compensating for minor focus shifts that may result over time or by stage movements. In this
manual, this method will be referred to as in-focus observation (or PFS observation
(NOTE 2)
).
NOTE 1: The boundary surface is also referred to as an “interface”.
NOTE 2: PFS = Perfect Focus System
In-focus observation workflow
Outline: Perform bright-field microscopy or phase contrast microscopy with the TI-ND6-PFS PFS
Motorized Nosepiece.
* To perform in-focus observation, you must register the objective information on the microscope.
For registration details, refer to Chapter 4, “Assembly.” This section describes the microscopy
procedures under the assumption that the objective information has already been registered.
1
Focus on the specimen with bright-field microscopy or
phase contrast microscopy.
¨ Page 50
2
Set up the perfect focus system. ¨ Page 50
3
Start the operation of the perfect focus system. ¨ Page 51
4
Observe the specimen. ¨ Page 51
5
End the operation of the perfect focus system. ¨ Page 52
6
End the observation. ¨ Page 52
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