Nikon Eclipse Ti-E User Manual

Chapter 2 Microscopy
2.3 Phase Contrast (Ph) Microscopy
40
3
Center the condenser annular diaphragm.
B
Coarse
Fine
ExFine
Epi Shutter
FL
Block
Refocus
Escape
PFS
OFFSET
OUT
DIC
HR
O
IC
MIR
R
OR
I
N
L8
0
EYE
ME
M
ORY
DI
S
P
L
A
Y
ON
Z
-
R
ES
ET
1X
1.5X
BRIGHTNESS
R1
0
0
L
100
FOCUS
PF
S
Centering the annular diaphragm
1. Move the Bertrand lens into the optical path
by moving the Bertrand lens in/out lever to
position “B”. Adjust the focus onto the
annular diaphragm image by rotating the
Bertrand lens focusing knob on the right side
of the eyepiece tube.
2. Using the provided hex screwdriver, rotate
the two annular diaphragm centering screws
on the condenser cassette, and adjust the
position of the annular diaphragm image so
that it overlaps with the phase contrast ring
image within the objective.
3. Move the Bertrand lens in/out lever back to
position “O”.
4
Observe the specimen.
Coarse
Fine
ExFine
Obj.
ON
OFF
6V30W
MAX.
12V100W
L80
EYE
DISPLAY
MEMORY
PFS
ON
RECALL
Z
-
RESET
BRIGHTNESS
R100
L100
FOCUS
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT
DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z
-
RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
1. Check that the aperture diaphragm of the
condenser is fully open.
2. Adjust the size of the field diaphragm image
to closely match the size of the field of view,
by rotating the field diaphragm knob on the
dia pillar illuminator.
3. Move the NCB11 filter on the dia pillar
illuminator out of the optical path, and move
the GIF filter into the optical path.
The GIF filter improves the contrast for phase
contrast microscopy.
4. Adjust the brightness for the field of view by
moving the ND filters on the dia pillar
illuminator in and out of the optical path.
If color reproducibility is not required, brightness
adjustment can be performed by changing the
lamp voltage with the brightness control knob on
the left side of the microscope.
5. Move the stage to bring the observation
target into the center of the field of view.
Adjust the focus by rotating the focus knobs.
3-1, 3-3
3-2
Overlap annular diaphragm image
onto phase contrast ring image within objective.
A
nnular
diaphragm
image
(4-4)
4-3, 4-4
4-2
4-1
4-5
Phase contrast
ring image

Nikon Eclipse Ti-E Specifications

General

Brand	Nikon
Model	Eclipse Ti-E
Category	Microscope
Language	English