Chapter 2 Microscopy
2.2 Bright-Field (BF) Microscopy
34
7
Adjust the diopters and the interpupillary distance.
Coarse
Fine
ExFine
Obj.
ON
OFF
6V30
W
M
AX.
12
V100W
L80
EYE
DISPLAY
MEMORY
PFS
ON
RECALL
Z
-
RESET
BRI
GHTNES
S
R100
L100
FOCUS
Diopter adjustment on eyepieces
Interpupillary adjustment on binocular
1. On each eyepiece, rotate the diopter
adjustment ring to align its lower end with the
marking on the eyepiece.
This will be the reference position for diopter
adjustment.
2. Move the 40x objective into the optical path
by pressing the Obj. switch.
3. Look into the left eyepiece with your left eye.
Adjust the focus onto the specimen by
rotating the focus knobs.
Use the Coarse/Fine/ExFine switches to change
the focus knob resolution for easier focus
adjustment.
4. Move the 10x objective into the optical path
by pressing the Obj. switch.
5. Look into the left eyepiece with your left eye.
Adjust the focus onto the specimen by
rotating the diopter adjustment ring on the
left eyepiece.
Do not touch the focus knobs at this time.
6. Repeat steps 2 through 5 two times.
7. Adjust the right eyepiece.
Repeat steps 2 through 6, but this time using the
right eyepiece instead of the left.
8. Adjust the interpupillary distance of the
binocular part to merge the two fields of view.
8
Re-adjust the focus.
B
Coarse
Fine
ExFine
Epi Shutter
FL Block
Refocus
Escape
PFS
OFFSET
OUT
DICHROIC MIRROR
IN
L80
EYE
MEMORY
DISPLAY
ON
Z
-
RESET
1X
1.5X
BRIGHTNESS
R100
L100
FOCUS
PFS
1. Look into the eyepiece. Move the stage to
bring the observation target into the center of
the field of view.
2. Focus on the target by rotating the focus
knobs.
Use the Coarse/Fine/ExFine switches to change
the focus knob resolution for easier focus
adjustment.
Markin
Adjust interpupillary
distance of binocular part
to merge fields of view.
7-1, 7-5,
7-8
7-2, 7-4
7-3
8-1
8-2
8-2
Diopte
adjustment ring
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