Chapter 3 Operation
3.11 Objective Operation
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3.11.5 Water Immersion Objectives
Figure 3-37 Water immersion objective (example)
Objectives with the “WI” label are water immersion
objectives. (Those with long operating distances are
for upright microscopes.)
When using a water immersion objective, fill the
space between the objective tip and the specimen
with deionized or distilled water.
Caution
• Do NOT use tap water. If tap water is used, impurities may adhere to and solidify on the
lens, causing the lens to become scratched when being cleaned.
• Plan Apo 60xWI (NA=1.2) is equipped with a correction ring to achieve optimal aberration
on cover glasses of different thicknesses. A reading of “17” on the scale indicates 0.17 mm.
When using a cover glass, measure the thickness of the cover glass with a micrometer, and
adjust the correction ring for the thickness for a more accurate correction.
“WI” marking
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